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Self-Assembled Hybrid Structures of DNA Block-copolymers and Nanoparticles with Enhanced DNA Binding and High Selectivity

Xi-Jun Chen, Brenda L. Sanchez-Gaytan, Sara E. N. Hayik, Michael Fryd, Bradford B. Wayland, and So-Jung Park

Schematic description and the fluorescence spectra showing the preferential binding of target DNA to DNA block-copolymer micellesSchematic description and the fluorescence spectra showing the preferential binding of target DNA to DNA block-copolymer micelles

DNA-functionalized nanoparticles are promising DNA probes for various biological and medical applications such as bio-imaging and sensing, medical diagnostics, and drug delivery. Here we developed a new general synthetic method to functionalize nanoparticles with a high density DNA layer, based on the self-assembly of DNA block-copolymers and nanoparticles.

Remarkably, the self-assembled hybrid structure possesses extremely high binding capability to complementary DNA even at very low salt concentrations, where plain DNA strands do not form duplex structure.

The hybrid DNA nanostructure recognizes complementary DNA in very high selectivity and can differentiate single base mismatches from complementary strands.

Given the high binding efficiency and selectivity, the hybrid material should be extremely effective in duplex DNA detection applications.

 

 

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