Single Molecule Tracking Using Split Images
Yujie Sun, Yale E. Goldman
3D optical microscopic tracking has been long pursued by biologists and material scientists as it provides valuable insights that are unavailable in conventional 2D imaging. We developed a 3D tracking technique that can track single molecules in 3D with high temporal and spatial resolutions.
We demonstrated the 3D tracking capability of Parallax by tracking myosin X motors walking along actin filaments suspended across microfabricated structures.
Parallax 3D tracking reveals that myosin X walks in a right-handed helical path along actin filaments
Parallax can also provide 3D profiles of more complex shapes such as actin filaments. Shown here are the 3D profiles of 4 segments of actins (color contours in the right image) that cross over microfeatures
Our work greatly advances 3D optical imaging techniques and will enhance cell biology and biophysics research.