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Fluorescence Enhancement from Individual Ribosomes Using Silver Nano-Structures in Single Molecule Study of Translation

Shashank Bharill, Chunlai Chen, Ben Stevens, Karol Gryczynski, Ignacy Gryczynski and Yale Goldman

Objectives:

Enhancement of the fluorescence signal from individual ribosomes during protein synthesis using silver nano–structures.

Silver colloids were deposited on the surface by electrostatic interaction and then ribosomes were attached using biotinylated mRNA.

Silver colloids were deposited on the surface by electrostatic interaction and then ribosomes were attached using biotinylated mRNA.

The measured fluorescence signals are from initiation complexes labeled with Cy5 dye (IC-Cy5) on glass slide with or without silver colloids. The intensity from individual ribosomes was 5-10 fold higher on colloidal surfaces than on plain glass.

The measured fluorescence signals are from initiation complexes labeled with Cy5 dye (IC-Cy5) on glass slide with or without silver colloids. The intensity from individual ribosomes was 5-10 fold higher on colloidal surfaces than on plain glass.

 

 

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