AFM and Multi-Color pol-TIRF Nano-Imaging: Multi-Motor Filament Example

Dennis Discher and Yale Goldman

Global objectives and specific goals of the project:

To obtain structural and mechanical information from biological nano-machines before/during/after adsorption, assembly, dissection, compression, and/or unfolding during cycles of mechano-chemical activity.

Scientific strategy, approach and uniqueness

No one in the world has reported single molecule fluorescence simultaneous with single molecule mechanical probing.