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AFM + Fluorescence Microscopy for Single Molecule Biophysics – Initial tests on Ribosomes

PI: Yale Goldman, Barry Cooperman, Dennis Discher

Single molecule fluorescence and AFM have separately emerged as two of the most critical tools for biophysics. We seek to combine the two methods to better understand RIBOSOMES, which are the cell’s complex protein synthesis ‘factories’.

Conclusions:

Dual modality nano-imaging presents problems in coupling such as optical or mechanical interference, but these problems appear solvable and should allow wide application. Our initial studies of Ribosomes illustrate this, and as we probe, impose and monitor conformations we already find that these complexes are surprisingly stiff.

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