Integration and Control of Molecular Motors
J.L. Ross, Y.E. Goldman, E. Holzbaur, M.O. Ostap
Global objectives and specific goals of the project: Understand the regulatory relationships and cooperation among the three molecular motor families, dynein, kinesin, and myosin
Scientific strategy, approach and uniqueness: Extend single molecule optical trap and TIRF microscopy to dynein, kinesin and to artificial cargos in vitro
Conclusion and impact: Dynein/dynactin complexes processively tanslocate in both directions along microtubules. Both dynein and kinesin can switch microtubules at crossings. Calmodulin light-chain regulation of unconventional myosins is mediated by a paradoxical increase in dissociation in a context of increased affinity, presumably by displacement along adjacent binding motifs.
Ross, J.L., Wallace, K., Shuman, H, Goldman, Y.E., and Holzbaur, E.L.F. Processive Bidirectional Motion of Dynein-Dynactin Complexes in Vitro Submitted to I, 2005.
Ross, J.L., Shuman,H., Holzbaur, E.L.F. and Goldman, Y.E. Track Switching and Crossing by Microtubule Motors, Biophys. J. (Abstr) In Press, 2006